Dataset Information

Identification and degradation characteristics of Bacillus cereus strain WD-2 isolated from prochloraz-manganese-contaminated soils.

ABSTRACT: The bacterial strain WD-2, which was capable of efficiently degrading prochloraz-manganese, was isolated from soil contaminated with prochloraz-manganese, selected through enrichment culturing and identified as Bacillus cereus. Test results indicated that the optimal temperature and pH for bacterial growth were 35-40°C and 7.0-8.0, respectively. The highest degradation rate was above 88-90% when the pH was 7.0~8.0 and reached a maximum value (90.7%) at approximately 8.0. In addition, the bacterium showed the greatest growth ability with an OD600 of 0.805 and the highest degradation rate (68.2%) when glucose was chosen as the carbon source, while the difference in nitrogen source had no obvious influence on bacterial growth. The degradation rate exceeded 80% when the NaCl concentration was 0~2% and the rate reached 89.2% at 1%. When the concentration was higher than 7%, the growth of WD-2 and the degradation of prochloraz-manganese were found to be inhibited, and the degradation rate was merely 8.5%. The results indicated that strain WD-2 was able to effectively degrade prochloraz-manganese and might contribute to the bioremediation of contaminated soils.

SUBMITTER: Jiang J

PROVIDER: S-EPMC6688804 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Identification and degradation characteristics of Bacillus cereus strain WD-2 isolated from prochloraz-manganese-contaminated soils.

Jiang Jie J Tang Meizhen M Chen Junfeng J Yang Yuewei Y

PloS one 20190809 8

The bacterial strain WD-2, which was capable of efficiently degrading prochloraz-manganese, was isolated from soil contaminated with prochloraz-manganese, selected through enrichment culturing and identified as Bacillus cereus. Test results indicated that the optimal temperature and pH for bacterial growth were 35-40°C and 7.0-8.0, respectively. The highest degradation rate was above 88-90% when the pH was 7.0~8.0 and reached a maximum value (90.7%) at approximately 8.0. In addition, the bacteri ...[more]

PMID: 31398235

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Project description:Gram-positive, spore-forming members of the Bacillus cereus group species complex are widespread in natural environments and display various degrees of pathogenicity. Recently, B. cereus group strain Bacillus mycoides Flugge ATCC 21929 was found to represent a novel lineage within the species complex, sharing a relatively low degree of genomic similarity with all B. cereus group genomes (average nucleotide identity [ANI] < 88). ATCC 21929 has been previously associated with the production of a patented antibiotic, antibiotic 60-6 (i.e., cerexin A); however, the virulence potential and growth characteristics of this lineage have never been assessed. Here, we provide an extensive genomic and phenotypic characterization of ATCC 21929, and we assess its pathogenic potential in vitro. ATCC 21929 most closely resembles Bacillus paramycoides NH24A2T (ANI and in silico DNA-DNA hybridization values of 86.70 and 34.10%, respectively). Phenotypically, ATCC 21929 does not possess cytochrome c oxidase activity and is able to grow at a range of temperatures between 15 and 43°C and a range of pH between 6 and 9. At 32°C, ATCC 21929 shows weak production of diarrheal enterotoxin hemolysin BL (Hbl) but no production of nonhemolytic enterotoxin (Nhe); at 37°C, neither Hbl nor Nhe is produced. Additionally, at 37°C, ATCC 21929 does not exhibit cytotoxic effects toward HeLa cells. With regard to fatty acid composition, ATCC 21929 has iso-C17:0 present in highest abundance. Based on the characterization provided here, ATCC 21929T (= PS00077AT = PS00077BT = PSU-0922T = BHPT) represents a novel effective B. cereus group species, which we propose as effective species "Bacillus clarus"IMPORTANCE The B. cereus group comprises numerous closely related lineages with various degrees of pathogenic potential and industrial relevance. Species-level taxonomic classification of B. cereus group strains is important for risk evaluation and communication but remains challenging. Biochemical and phenotypic assays are often used to assign B. cereus group strains to species but are insufficient for accurate taxonomic classification on a genomic scale. Here, we show that antibiotic-producing ATCC 21929 represents a novel lineage within the B. cereus group that, by all metrics used to delineate prokaryotic species, exemplifies a novel effective species. Furthermore, we show that ATCC 21929 is incapable of producing enterotoxins Hbl and Nhe or exhibiting cytotoxic effects on HeLa cells at human body temperature in vitro These results provide greater insight into the genomic and phenotypic diversity of the B. cereus group and may be leveraged to inform future public health and food safety efforts.

Dataset Information

Identification and degradation characteristics of Bacillus cereus strain WD-2 isolated from prochloraz-manganese-contaminated soils.

Publications

Identification and degradation characteristics of Bacillus cereus strain WD-2 isolated from prochloraz-manganese-contaminated soils.

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