Dataset Information

Biodegradation of Allethrin by a Novel Fungus Fusarium proliferatum Strain CF2, Isolated from Contaminated Soils.

ABSTRACT: Continuous use of allethrin has resulted in heavy environmental contamination and has raised public concern about its impact on human health, yet little is known about the kinetics and microbial degradation of this pesticide. This study reported the degradation kinetics in a novel fungal strain, Fusarium proliferatum CF2, isolated from contaminated agricultural fields. Strain CF2 utilized 50 mg·L^-1 of allethrin as the sole carbon source for growth in minimal salt medium and tolerated high concentrations of allethrin of up to 1000 mg·L^-1. The optimum degradation conditions for strain CF2 were determined to be a temperature of 26 °C and pH 6.0 using response surface methodology. Under optimum conditions, strain CF2 completely degraded allethrin within 144 hours. The degradation kinetics of allethrin followed first order reaction kinetics. Kinetics analysis showed that its half-life was substantially reduced by 507.1 hours, as compared to the uninoculated control. This study provides new insights into the microbial degradation of allethrin with fungal F. proliferatum CF2.

SUBMITTER: Bhatt P

PROVIDER: S-EPMC7232317 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

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Biodegradation of Allethrin by a Novel Fungus Fusarium proliferatum Strain CF2, Isolated from Contaminated Soils.

Bhatt Pankaj P Zhang Wenping W Lin Ziqiu Z Pang Shimei S Huang Yaohua Y Chen Shaohua S

Microorganisms 20200420 4

Continuous use of allethrin has resulted in heavy environmental contamination and has raised public concern about its impact on human health, yet little is known about the kinetics and microbial degradation of this pesticide. This study reported the degradation kinetics in a novel fungal strain, Fusarium proliferatum CF2, isolated from contaminated agricultural fields. Strain CF2 utilized 50 mg·L-1 of allethrin as the sole carbon source for growth in minimal salt medium and tol ...[more]

PMID: 32325934

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Project description:Six chlorpyrifos-degrading bacteria were isolated from an Australian soil and compared by biochemical and molecular methods. The isolates were indistinguishable, and one (strain B-14) was selected for further analysis. This strain showed greatest similarity to members of the order Enterobacteriales and was closest to members of the Enterobacter asburiae group. The ability of the strain to mineralize chlorpyrifos was investigated under different culture conditions, and the strain utilized chlorpyrifos as the sole source of carbon and phosphorus. Studies with ring or uniformly labeled [(14)C]chlorpyrifos in liquid culture demonstrated that the isolate hydrolyzed chlorpyrifos to diethylthiophospshate (DETP) and 3, 5, 6-trichloro-2-pyridinol, and utilized DETP for growth and energy. The isolate was found to possess mono- and diphosphatase activities along with a phosphotriesterase activity. Addition of other sources of carbon (glucose and succinate) resulted in slowing down of the initial rate of degradation of chlorpyrifos. The isolate degraded the DETP-containing organophosphates parathion, diazinon, coumaphos, and isazofos when provided as the sole source of carbon and phosphorus, but not fenamiphos, fonofos, ethoprop, and cadusafos, which have different side chains. Studies of the molecular basis of degradation suggested that the degrading ability could be polygenic and chromosome based. Further studies revealed that the strain possessed a novel phosphotriesterase enzyme system, as the gene coding for this enzyme had a different sequence from the widely studied organophosphate-degrading gene (opd). The addition of strain B-14 (10(6) cells g(-1)) to soil with a low indigenous population of chlorpyrifos-degrading bacteria treated with 35 mg of chlorpyrifos kg(-1) resulted in a higher degradation rate than was observed in noninoculated soils. These results highlight the potential of this bacterium to be used in the cleanup of contaminated pesticide waste in the environment.

Dataset Information

Biodegradation of Allethrin by a Novel Fungus Fusarium proliferatum Strain CF2, Isolated from Contaminated Soils.

Publications

Biodegradation of Allethrin by a Novel Fungus <i>Fusarium proliferatum</i> Strain CF2, Isolated from Contaminated Soils.

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