Ontology highlight
ABSTRACT:
SUBMITTER: Kanonenberg K
PROVIDER: S-EPMC6689329 | biostudies-literature | 2019 Aug
REPOSITORIES: biostudies-literature
Kanonenberg Kerstin K Royes Jorge J Kedrov Alexej A Poschmann Gereon G Angius Federica F Solgadi Audrey A Spitz Olivia O Kleinschrodt Diana D Stühler Kai K Miroux Bruno B Schmitt Lutz L
Microbial cell factories 20190810 1
<h4>Background</h4>The overexpression and purification of membrane proteins is a bottleneck in biotechnology and structural biology. E. coli remains the host of choice for membrane protein production. To date, most of the efforts have focused on genetically tuning of expression systems and shaping membrane composition to improve membrane protein production remained largely unexplored.<h4>Results</h4>In E. coli C41(DE3) strain, we deleted two transporters involved in fatty acid metabolism (OmpF a ...[more]