Ontology highlight
ABSTRACT:
SUBMITTER: Wang Q
PROVIDER: S-EPMC6698433 | biostudies-literature | 2020 Feb
REPOSITORIES: biostudies-literature
Wang Qian Q Bhattacharya Soumyaroop S Mereness Jared A JA Anderson Christopher C Lillis Jacquelyn A JA Misra Ravi S RS Romas Stephen S Huyck Heidie H Howell Amanda A Bandyopadhyay Gautam G Donlon Kathy K Myers Jason R JR Ashton John J Pryhuber Gloria S GS Mariani Thomas J TJ
Pediatric research 20190218 3
<h4>Background</h4>Current in vitro human lung epithelial cell models derived from adult tissues may not accurately represent all attributes that define homeostatic and disease mechanisms relevant to the pediatric lung.<h4>Methods</h4>We report methods for growing and differentiating primary Pediatric Human Lung Epithelial (PHLE) cells from organ donor infant lung tissues. We use immunohistochemistry, flow cytometry, quantitative RT-PCR, and single cell RNA sequencing (scRNAseq) analysis to char ...[more]