FLASH: a next-generation CRISPR diagnostic for multiplexed detection of antimicrobial resistance sequences.
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ABSTRACT:
SUBMITTER: Quan J
PROVIDER: S-EPMC6698650 | biostudies-literature | 2019 Aug
REPOSITORIES: biostudies-literature
Publications
FLASH: a next-generation CRISPR diagnostic for multiplexed detection of antimicrobial resistance sequences.
Quan Jenai J Langelier Charles C Kuchta Alison A Batson Joshua J Teyssier Noam N Lyden Amy A Caldera Saharai S McGeever Aaron A Dimitrov Boris B King Ryan R Wilheim Jordan J Murphy Maxwell M Ares Lara Pesce LP Travisano Katherine A KA Sit Rene R Amato Roberto R Mumbengegwi Davis R DR Smith Jennifer L JL Bennett Adam A Gosling Roly R Mourani Peter M PM Calfee Carolyn S CS Neff Norma F NF Chow Eric D ED Kim Peter S PS Greenhouse Bryan B DeRisi Joseph L JL Crawford Emily D ED
Nucleic acids research 20190801 14
The growing prevalence of deadly microbes with resistance to previously life-saving drug therapies is a dire threat to human health. Detection of low abundance pathogen sequences remains a challenge for metagenomic Next Generation Sequencing (NGS). We introduce FLASH (Finding Low Abundance Sequences by Hybridization), a next-generation CRISPR/Cas9 diagnostic method that takes advantage of the efficiency, specificity and flexibility of Cas9 to enrich for a programmed set of sequences. FLASH-NGS a ...[more]
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