Proteomics

Dataset Information

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Next-generation diagnostic biomarkers for vivax malaria


ABSTRACT: The global impact of Plasmodium vivax has been largely underestimated for several decades due to lower mortality rates compared to P. falciparum. However, in recent times, the parasite has become a serious threat to public health due to its ability to cause severe malaria with fatal outcomes. Its unique biology makes it resilient to control measures and poses a challenge to available diagnostic methods. Diagnosis by RDTs is further restricted due to inadequate P. vivax specific antigens for species identification. Therefore, there is an urgent need to develop tests that employ antigens unique to the parasite. This study represents the first in-depth proteomics analysis of human plasma and parasite isolates to identify P. vivax protein biomarkers that can be tested for use in RDTs while developing diagnostics for malaria. Here we report 39 P. vivax proteins in human plasma and 103 highly expressed P. vivax proteins from parasite isolates with high confidence. Interestingly, five proteins, found to be unique to P. vivax were detected in both sources, representing the best candidates for evaluation as diagnostic markers. Moreover, targeted proteomics assays were used to validate some of these proteins. This study represents the first step in the development of new diagnostic assays for P. vivax malaria.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Plasmodium Vivax

TISSUE(S): Blood Cell, Blood Plasma, Blood

DISEASE(S): Plasmodium Vivax Malaria

SUBMITTER: Shalini Aggarwal  

LAB HEAD: Prof. Sanjeeva Srivastava

PROVIDER: PXD015165 | Pride | 2020-01-30

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
AV_Parasite_10_Fr1.mzML Mzml
AV_Parasite_10_Fr2.mzML Mzml
AV_Parasite_10_Fr3.mzML Mzml
AV_Parasite_10_Fr4.mzML Mzml
AV_Parasite_10_Fr5.mzML Mzml
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