Ontology highlight
ABSTRACT:
SUBMITTER: Hohl A
PROVIDER: S-EPMC6700097 | biostudies-literature | 2019 Aug
REPOSITORIES: biostudies-literature
Scientific reports 20190819 1
The Pyrrolysyl-tRNA synthetase (PylRS) and its cognate tRNA<sup>Pyl</sup> are extensively used to add non-canonical amino acids (ncAAs) to the genetic code of bacterial and eukaryotic cells. However, new ncAAs often require a cumbersome de novo engineering process to generate an appropriate PylRS/tRNA<sup>Pyl</sup> pair. We here report a strategy to predict a PylRS variant with novel properties. The designed polyspecific PylRS variant HpRS catalyzes the aminoacylation of 31 structurally diverse ...[more]