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Spatiotemporal dissection of the trans-Golgi network in budding yeast.


ABSTRACT: The trans-Golgi network (TGN) acts as a sorting hub for membrane traffic. It receives newly synthesized and recycled proteins, and sorts and delivers them to specific targets such as the plasma membrane, endosomes and lysosomes/vacuoles. Accumulating evidence suggests that the TGN is generated from the trans-most cisterna of the Golgi by maturation, but the detailed transition processes remain obscure. Here, we examine spatiotemporal assembly dynamics of various Golgi/TGN-resident proteins in budding yeast by high-speed and high-resolution spinning-disk confocal microscopy. The Golgi-TGN transition gradually proceeds via at least three successive stages: the 'Golgi stage' where glycosylation occurs; the 'early TGN stage', which receives retrograde traffic; and the 'late TGN stage', where transport carriers are produced. During the stage transition periods, earlier and later markers are often compartmentalized within a cisterna. Furthermore, for the late TGN stage, various types of coat/adaptor proteins exhibit distinct assembly patterns. Taken together, our findings characterize the identity of the TGN as a membrane compartment that is structurally and functionally distinguishable from the Golgi.This article has an associated First Person interview with the first author of the paper.

SUBMITTER: Tojima T 

PROVIDER: S-EPMC6703704 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature

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Spatiotemporal dissection of the <i>trans</i>-Golgi network in budding yeast.

Tojima Takuro T   Suda Yasuyuki Y   Ishii Midori M   Kurokawa Kazuo K   Nakano Akihiko A  

Journal of cell science 20190802 15


The <i>trans</i>-Golgi network (TGN) acts as a sorting hub for membrane traffic. It receives newly synthesized and recycled proteins, and sorts and delivers them to specific targets such as the plasma membrane, endosomes and lysosomes/vacuoles. Accumulating evidence suggests that the TGN is generated from the <i>trans</i>-most cisterna of the Golgi by maturation, but the detailed transition processes remain obscure. Here, we examine spatiotemporal assembly dynamics of various Golgi/TGN-resident  ...[more]

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