Ontology highlight
ABSTRACT:
SUBMITTER: Wasserman MR
PROVIDER: S-EPMC6705614 | biostudies-literature | 2019 Jul
REPOSITORIES: biostudies-literature
Wasserman Michael R MR Schauer Grant D GD O'Donnell Michael E ME Liu Shixin S
Cell 20190701 3
The eukaryotic replicative helicase CMG is a closed ring around double-stranded (ds)DNA at origins yet must transition to single-stranded (ss)DNA for helicase action. CMG must also handle repair intermediates, such as reversed forks that lack ssDNA. Here, using correlative single-molecule fluorescence and force microscopy, we show that CMG harbors a ssDNA gate that enables transitions between ss and dsDNA. When coupled to DNA polymerase, CMG remains on ssDNA, but when uncoupled, CMG employs this ...[more]