Inhibition of amyloid beta toxicity in zebrafish with a chaperone-gold nanoparticle dual strategy.

ABSTRACT: Alzheimer's disease (AD) is the most prevalent form of neurodegenerative disorders, yet no major breakthroughs have been made in AD human trials and the disease remains a paramount challenge and a stigma in medicine. Here we eliminate the toxicity of amyloid beta (A?) in a facile, high-throughput zebrafish (Danio rerio) model using casein coated-gold nanoparticles (?Cas AuNPs). ?Cas AuNPs in systemic circulation translocate across the blood brain barrier of zebrafish larvae and sequester intracerebral A?₄₂ and its elicited toxicity in a nonspecific, chaperone-like manner. This is evidenced by behavioral pathology, reactive oxygen species and neuronal dysfunction biomarkers assays, complemented by brain histology and inductively coupled plasma-mass spectroscopy. We further demonstrate the capacity of ?Cas AuNPs in recovering the mobility and cognitive function of adult zebrafish exposed to A?. This potent, safe-to-use, and easy-to-apply nanomedicine may find broad use for eradicating toxic amyloid proteins implicated in a range of human diseases.

SUBMITTER: Javed I 

PROVIDER: S-EPMC6706415 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature