Unknown

Dataset Information

0

Self-organized formation of developing appendages from murine pluripotent stem cells.

ABSTRACT: Limb development starts with the formation of limb buds (LBs), which consist of tissues from two different germ layers; the lateral plate mesoderm-derived mesenchyme and ectoderm-derived surface epithelium. Here, we report means for induction of an LB-like mesenchymal/epithelial complex tissues from murine pluripotent stem cells (PSCs) in vitro. The LB-like tissues selectively differentiate into forelimb- or hindlimb-type mesenchymes, depending on a concentration of retinoic acid. Comparative transcriptome analysis reveals that the LB-like tissues show similar gene expression pattern to that seen in LBs. We also show that manipulating BMP signaling enables us to induce a thickened epithelial structure similar to the apical ectodermal ridge. Finally, we demonstrate that the induced tissues can contribute to endogenous digit tissue after transplantation. This PSC technology offers a first step for creating an artificial limb bud in culture and might open the door to inducing other mesenchymal/epithelial complex tissues from PSCs.

SUBMITTER: Mori S 

PROVIDER: S-EPMC6707191 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Self-organized formation of developing appendages from murine pluripotent stem cells.

Mori Shunsuke S   Sakakura Eriko E   Tsunekawa Yuji Y   Hagiwara Masaya M   Suzuki Takayuki T   Eiraku Mototsugu M  

Nature communications 20190823 1

Limb development starts with the formation of limb buds (LBs), which consist of tissues from two different germ layers; the lateral plate mesoderm-derived mesenchyme and ectoderm-derived surface epithelium. Here, we report means for induction of an LB-like mesenchymal/epithelial complex tissues from murine pluripotent stem cells (PSCs) in vitro. The LB-like tissues selectively differentiate into forelimb- or hindlimb-type mesenchymes, depending on a concentration of retinoic acid. Comparative tr  ...[more]

Similar Datasets

Unknown Self-organized vascular networks from human pluripotent stem cells in a synthetic matrix.
| S-EPMC3732921 | biostudies-literature
Unknown Self-organized amniogenesis by human pluripotent stem cells in a biomimetic implantation-like niche.
| S-EPMC5374007 | biostudies-literature
Unknown Differential coupling of self-renewal signaling pathways in murine induced pluripotent stem cells.
| S-EPMC3264619 | biostudies-literature
Methylation profiling YAP1 is essential for self-organized differentiation of pluripotent stem cells
2023-02-17 | GSE214403 | GEO
Unknown Self-organized criticality in developing neuronal networks.
| S-EPMC2996321 | biostudies-literature
Transcriptomics YAP1 is essential for self-organized differentiation of pluripotent stem cells [RNAseq]
2023-02-17 | GSE214401 | GEO
Methylation profiling YAP1 is essential for self-organized differentiation of pluripotent stem cells [DNAm]
2023-02-17 | GSE214400 | GEO
Unknown Evidence of Adult Features and Functions of Hepatocytes Differentiated from Human Induced Pluripotent Stem Cells and Self-Organized as Organoids.
| S-EPMC8834365 | biostudies-literature
Unknown Lineage conversion of murine extraembryonic trophoblast stem cells to pluripotent stem cells.
| S-EPMC3126346 | biostudies-literature
Unknown Developing high-fidelity hepatotoxicity models from pluripotent stem cells.
| S-EPMC3697818 | biostudies-other