Dataset Information

Automated real-time monitoring of human pluripotent stem cell aggregation in stirred tank reactors.

ABSTRACT: The culture of human induced pluripotent stem cells (hiPSCs) at large scale becomes feasible with the aid of scalable suspension setups in continuously stirred tank reactors (CSTRs). Innovative monitoring options and emerging automated process control strategies allow for the necessary highly defined culture conditions. Next to standard process characteristics such as oxygen consumption, pH, and metabolite turnover, a reproducible and steady formation of hiPSC aggregates is vital for process scalability. In this regard, we developed a hiPSC-specific suspension culture unit consisting of a fully monitored CSTR system integrated into a custom-designed and fully automated incubator. As a step towards cost-effective hiPSC suspension culture and to pave the way for flexibility at a large scale, we constructed and utilized tailored miniature CSTRs that are largely made from three-dimensional (3D) printed polylactic acid (PLA) filament, which is a low-cost material used in fused deposition modelling. Further, the monitoring tool for hiPSC suspension cultures utilizes in situ microscopic imaging to visualize hiPSC aggregation in real-time to a statistically significant degree while omitting the need for time-intensive sampling. Suitability of our culture unit, especially concerning the developed hiPSC-specific CSTR system, was proven by demonstrating pluripotency of CSTR-cultured hiPSCs at RNA (including PluriTest) and protein level.

SUBMITTER: Schwedhelm I

PROVIDER: S-EPMC6707254 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature

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Automated real-time monitoring of human pluripotent stem cell aggregation in stirred tank reactors.

Schwedhelm Ivo I Zdzieblo Daniela D Appelt-Menzel Antje A Berger Constantin C Schmitz Tobias T Schuldt Bernhard B Franke Andre A Müller Franz-Josef FJ Pless Ole O Schwarz Thomas T Wiedemann Philipp P Walles Heike H Hansmann Jan J

Scientific reports 20190823 1

The culture of human induced pluripotent stem cells (hiPSCs) at large scale becomes feasible with the aid of scalable suspension setups in continuously stirred tank reactors (CSTRs). Innovative monitoring options and emerging automated process control strategies allow for the necessary highly defined culture conditions. Next to standard process characteristics such as oxygen consumption, pH, and metabolite turnover, a reproducible and steady formation of hiPSC aggregates is vital for process sca ...[more]

PMID: 31444389

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Dataset Information

Automated real-time monitoring of human pluripotent stem cell aggregation in stirred tank reactors.

Publications

Automated real-time monitoring of human pluripotent stem cell aggregation in stirred tank reactors.

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