Inverse problem analysis of pluripotent stem cell aggregation dynamics in stirred-suspension cultures.

ABSTRACT: The cultivation of stem cells as aggregates in scalable bioreactor cultures is an appealing modality for the large-scale manufacturing of stem cell products. Aggregation phenomena are central to such bioprocesses affecting the viability, proliferation and differentiation trajectory of stem cells but a quantitative framework is currently lacking. A population balance equation (PBE) model was used to describe the temporal evolution of the embryonic stem cell (ESC) cluster size distribution by considering collision-induced aggregation and cell proliferation in a stirred-suspension vessel. For ESC cultures at different agitation rates, the aggregation kernel representing the aggregation dynamics was successfully recovered as a solution of the inverse problem. The rate of change of the average

SUBMITTER: Rostami MR 

PROVIDER: S-EPMC4520320 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature