Ontology highlight
ABSTRACT:
SUBMITTER: Hua L
PROVIDER: S-EPMC6710646 | biostudies-literature | 2019 Aug
REPOSITORIES: biostudies-literature
Plant direct 20190827 8
Laser Capture Microdissection is a powerful tool that allows thin slices of specific cell types to be separated from one another. However, the most commonly used protocol, which involves embedding tissue in paraffin wax, results in severely degraded RNA. Yields from low abundance cell types of leaves are particularly compromised. We reasoned that the relatively high temperature used for sample embedding, and aqueous conditions associated with sample preparation prior to microdissection contribut ...[more]