An Optimized Protocol of Laser Capture Microdissection for Tissue-Specific RNA Profiling in a Radish Tap Root.
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ABSTRACT:
SUBMITTER: Choe G
PROVIDER: S-EPMC7756924 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
Publications
An Optimized Protocol of Laser Capture Microdissection for Tissue-Specific RNA Profiling in a Radish Tap Root.
Choe Goh G Hoang Nam V NV Lee Ji-Young JY
STAR protocols 20200917 3
Laser capture microdissection (LCM) coupled with transcriptome profiling is a powerful technique that allows for tissue-specific gene expression analysis in a complex system. One major challenge in using this technique is to obtain RNA without compromising its integrity. Here, we present a protocol optimized for radish root tissue sections using Steedman's wax embedding to obtain high-quality RNA suitable for next-generation sequencing analysis. For complete details on the use and execution of t ...[more]
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