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Meiotic cellular rejuvenation is coupled to nuclear remodeling in budding yeast.


ABSTRACT: Production of healthy gametes in meiosis relies on the quality control and proper distribution of both nuclear and cytoplasmic contents. Meiotic differentiation naturally eliminates age-induced cellular damage by an unknown mechanism. Using time-lapse fluorescence microscopy in budding yeast, we found that nuclear senescence factors - including protein aggregates, extrachromosomal ribosomal DNA circles, and abnormal nucleolar material - are sequestered away from chromosomes during meiosis II and subsequently eliminated. A similar sequestration and elimination process occurs for the core subunits of the nuclear pore complex in both young and aged cells. Nuclear envelope remodeling drives the formation of a membranous compartment containing the sequestered material. Importantly, de novo generation of plasma membrane is required for the sequestration event, preventing the inheritance of long-lived nucleoporins and senescence factors into the newly formed gametes. Our study uncovers a new mechanism of nuclear quality control and provides insight into its function in meiotic cellular rejuvenation.

SUBMITTER: King GA 

PROVIDER: S-EPMC6711709 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature

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Meiotic cellular rejuvenation is coupled to nuclear remodeling in budding yeast.

King Grant A GA   Goodman Jay S JS   Schick Jennifer G JG   Chetlapalli Keerthana K   Jorgens Danielle M DM   McDonald Kent L KL   Ünal Elçin E  

eLife 20190809


Production of healthy gametes in meiosis relies on the quality control and proper distribution of both nuclear and cytoplasmic contents. Meiotic differentiation naturally eliminates age-induced cellular damage by an unknown mechanism. Using time-lapse fluorescence microscopy in budding yeast, we found that nuclear senescence factors - including protein aggregates, extrachromosomal ribosomal DNA circles, and abnormal nucleolar material - are sequestered away from chromosomes during meiosis II and  ...[more]

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