Unknown

Dataset Information

0

Role of C/EBP-? in Methamphetamine-Mediated Microglial Apoptosis.


ABSTRACT: Methamphetamine (MA) is a widely abused psychoactive drug that primarily damages the nervous system. However, the involvement of MA in the survival of microglia remains poorly understood. CCAAT-enhancer binding protein (C/EBP-?) is a transcription factor and an important regulator of cell apoptosis. Lipocalin2 (lcn2) is a known apoptosis inducer and is involved in many cell death processes. We hypothesized that C/EBP-? is involved in MA-induced lcn2-mediated microglial apoptosis. To test this hypothesis, we measured the protein expression of C/EBP-? after MA treatment and evaluated the effects of silencing C/EBP-? or lcn2 on MA-induced apoptosis in BV-2 cells and the mouse striatum after intrastriatal MA injection. MA exposure increased the expression of C/EBP-? and stimulated the lcn2-mediated modulation of apoptosis. Moreover, silencing the C/EBP-?-dependent lcn2 upregulation reversed the MA-induced microglial apoptosis. The in vivo relevance of these findings was confirmed in mouse models, which demonstrated that the microinjection of anti-C/EBP-? into the striatum ameliorated the MA-induced decrease survival of microglia. These findings provide a new insight regarding the specific contributions of C/EBP-?-lcn2 to microglial survival in the context of MA abuse.

SUBMITTER: Chen X 

PROVIDER: S-EPMC6712175 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

altmetric image

Publications

Role of C/EBP-β in Methamphetamine-Mediated Microglial Apoptosis.

Chen Xuebing X   Lu Jiancong J   Zhao Xu X   Chen Chuanxiang C   Qiao Dongfang D   Wang Huijun H   Yue Xia X  

Frontiers in cellular neuroscience 20190821


Methamphetamine (MA) is a widely abused psychoactive drug that primarily damages the nervous system. However, the involvement of MA in the survival of microglia remains poorly understood. CCAAT-enhancer binding protein (C/EBP-β) is a transcription factor and an important regulator of cell apoptosis. Lipocalin2 (lcn2) is a known apoptosis inducer and is involved in many cell death processes. We hypothesized that C/EBP-β is involved in MA-induced lcn2-mediated microglial apoptosis. To test this hy  ...[more]

Similar Datasets

| S-EPMC5082785 | biostudies-literature
| S-EPMC5551554 | biostudies-literature
| S-EPMC4833032 | biostudies-literature
| S-EPMC3824683 | biostudies-literature
| S-EPMC5571106 | biostudies-literature
| S-EPMC11282193 | biostudies-literature
| S-EPMC5639602 | biostudies-literature
| S-EPMC3563992 | biostudies-literature
| S-EPMC4340131 | biostudies-literature
| S-EPMC3001000 | biostudies-literature