Ontology highlight
ABSTRACT:
SUBMITTER: Sorensen DM
PROVIDER: S-EPMC6724510 | biostudies-literature | 2019 Apr
REPOSITORIES: biostudies-literature
Molecular biology of the cell 20190220 9
P5A ATPases are expressed in the endoplasmic reticulum (ER) of all eukaryotic cells, and their disruption results in severe ER stress. However, the function of these ubiquitous membrane proteins, which belong to the P-type ATPase superfamily, is unknown. We purified a functional tagged version of the Saccharomyces cerevisiae P5A ATPase Spf1p and observed that the ATP hydrolytic activity of the protein is stimulated by phosphatidylinositol 4-phosphate (PI4P). Furthermore, SPF1 exhibited negative ...[more]