Ontology highlight
ABSTRACT:
SUBMITTER: Grunewald J
PROVIDER: S-EPMC6730565 | biostudies-literature | 2019 Sep
REPOSITORIES: biostudies-literature
Grünewald Julian J Zhou Ronghao R Iyer Sowmya S Lareau Caleb A CA Garcia Sara P SP Aryee Martin J MJ Joung J Keith JK
Nature biotechnology 20190902 9
Cytosine or adenine base editors (CBEs or ABEs) can introduce specific DNA C-to-T or A-to-G alterations<sup>1-4</sup>. However, we recently demonstrated that they can also induce transcriptome-wide guide-RNA-independent editing of RNA bases<sup>5</sup>, and created selective curbing of unwanted RNA editing (SECURE)-BE3 variants that have reduced unwanted RNA-editing activity<sup>5</sup>. Here we describe structure-guided engineering of SECURE-ABE variants with reduced off-target RNA-editing acti ...[more]