Ontology highlight
ABSTRACT:
SUBMITTER: Tycko J
PROVIDER: S-EPMC6731277 | biostudies-literature | 2019 Sep
REPOSITORIES: biostudies-literature
Nature communications 20190906 1
Pooled CRISPR-Cas9 screens are a powerful method for functionally characterizing regulatory elements in the non-coding genome, but off-target effects in these experiments have not been systematically evaluated. Here, we investigate Cas9, dCas9, and CRISPRi/a off-target activity in screens for essential regulatory elements. The sgRNAs with the largest effects in genome-scale screens for essential CTCF loop anchors in K562 cells were not single guide RNAs (sgRNAs) that disrupted gene expression ne ...[more]