Unknown

Dataset Information

0

MicroRNA-10a-5p regulates macrophage polarization and promotes therapeutic adipose tissue remodeling.


ABSTRACT: OBJECTIVE:This study investigated the role of microRNAs generated from adipose tissue macrophages (ATMs) during adipose tissue remodeling induced by pharmacological and nutritional stimuli. METHODS:Macrophage-specific Dicer knockout (KO) mice were used to determine the roles of microRNA generated in macrophages in adipose tissue remodeling induced by the ?3-adrenergic receptor agonist CL316,243 (CL). RNA-seq was performed to characterize microRNA and mRNA expression profiles in isolated macrophages and PDGFR?+ adipocyte stem cells (ASCs). The role of miR-10a-5p was further investigated in cell culture, and in adipose tissue remodeling induced by CL treatment and high fat feeding. RESULTS:Macrophage-specific deletion of Dicer elevated pro-inflammatory gene expression and prevented CL-induced de novo beige adipogenesis in gonadal white adipose tissue (gWAT). Co-culture of ASCs with ATMs of wild type mice promoted brown adipocyte gene expression upon differentiation, but co-culture with ATMs of Dicer KO mice did not. Bioinformatic analysis of RNA expression profiles identified miR-10a-5p as a potential regulator of inflammation and differentiation in ATMs and ASCs, respectively. CL treatment increased levels of miR-10a-5p in ATMs and ASCs in gWAT. Interestingly, CL treatment elevated levels of pre-mir-10a in ATMs but not in ASCs, suggesting possible transfer from ATMs to ASCs. Elevating miR-10a-5p levels inhibited proinflammatory gene expression in cultured RAW 264.7 macrophages and promoted the differentiation of C3H10T1/2 cells into brown adipocytes. Furthermore, treatment with a miR-10a-5p mimic in vivo rescued CL-induced beige adipogenesis in Dicer KO mice. High fat feeding reduced miR-10a-5p levels in ATMs of gWAT, and treatment of mice with a miR-10a-5p mimic suppressed pro-inflammatory responses, promoted the appearance of new white adipocytes in gWAT, and improved systemic glucose tolerance. CONCLUSIONS:These results demonstrate an important role of macrophage-generated microRNAs in adipogenic niches and identify miR-10a-5p as a key regulator that reduces adipose tissue inflammation and promotes therapeutic adipogenesis.

SUBMITTER: Cho YK 

PROVIDER: S-EPMC6734158 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature

altmetric image

Publications

MicroRNA-10a-5p regulates macrophage polarization and promotes therapeutic adipose tissue remodeling.

Cho Yoon Keun YK   Son Yeonho Y   Kim Sang-Nam SN   Song Hyun-Doo HD   Kim Minsu M   Park Ji-Hyun JH   Jung Young-Suk YS   Ahn Sang-Yeop SY   Saha Abhirup A   Granneman James G JG   Lee Yun-Hee YH  

Molecular metabolism 20190827


<h4>Objective</h4>This study investigated the role of microRNAs generated from adipose tissue macrophages (ATMs) during adipose tissue remodeling induced by pharmacological and nutritional stimuli.<h4>Methods</h4>Macrophage-specific Dicer knockout (KO) mice were used to determine the roles of microRNA generated in macrophages in adipose tissue remodeling induced by the β3-adrenergic receptor agonist CL316,243 (CL). RNA-seq was performed to characterize microRNA and mRNA expression profiles in is  ...[more]

Similar Datasets

| S-EPMC3679284 | biostudies-literature
| S-EPMC2519760 | biostudies-literature
| S-EPMC4811113 | biostudies-literature
| S-EPMC5002490 | biostudies-literature
| S-EPMC10582718 | biostudies-literature
| S-EPMC6514622 | biostudies-literature
| S-EPMC9085364 | biostudies-literature
| S-EPMC10243739 | biostudies-literature
| S-EPMC10861897 | biostudies-literature
| S-EPMC10006517 | biostudies-literature