Ontology highlight
ABSTRACT:
SUBMITTER: Forsberg KJ
PROVIDER: S-EPMC6739867 | biostudies-literature | 2019 Sep
REPOSITORIES: biostudies-literature
Forsberg Kevin J KJ Bhatt Ishan V IV Schmidtke Danica T DT Javanmardi Kamyab K Dillard Kaylee E KE Stoddard Barry L BL Finkelstein Ilya J IJ Kaiser Brett K BK Malik Harmit S HS
eLife 20190910
CRISPR-Cas systems protect bacteria and archaea from phages and other mobile genetic elements, which use small anti-CRISPR (Acr) proteins to overcome CRISPR-Cas immunity. Because Acrs are challenging to identify, their natural diversity and impact on microbial ecosystems are underappreciated. To overcome this discovery bottleneck, we developed a high-throughput functional selection to isolate ten DNA fragments from human oral and fecal metagenomes that inhibit <i>Streptococcus pyogenes</i> Cas9 ...[more]