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Single-cell microarray analysis in hippocampus CA1: demonstration and validation of cellular heterogeneity.

ABSTRACT: Laser capture microdissection in combination with microarrays allows for the expression analysis of thousands of genes in selected cells. Here we describe single-cell gene expression profiling of CA1 neurons in the rat hippocampus using a combination of laser capture, T7 RNA amplification, and cDNA microarray analysis. Subsequent cluster analysis of the microarray data identified two different cell types: pyramidal neurons and an interneuron. Cluster analysis also revealed differences among the pyramidal neurons, indicating that even a single cell type in vivo is not a homogeneous population of cells at the gene expression level. Microarray data were confirmed by quantitative RT-PCR and in situ hybridization. We also report on the reproducibility and sensitivity of this combination of methods. Single-cell gene expression profiling offers a powerful tool to tackle the complexity of the mammalian brain.

SUBMITTER: Kamme F

PROVIDER: S-EPMC6742179 | biostudies-literature | 2003 May

REPOSITORIES: biostudies-literature

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Single-cell microarray analysis in hippocampus CA1: demonstration and validation of cellular heterogeneity.

Kamme Fredrik F Salunga Ranelle R Yu Jingxue J Tran Da-Thao DT Zhu Jessica J Luo Lin L Bittner Anton A Guo Hong-Qing HQ Miller Nancy N Wan Jackson J Erlander Mark M

The Journal of neuroscience : the official journal of the Society for Neuroscience 20030501 9

Laser capture microdissection in combination with microarrays allows for the expression analysis of thousands of genes in selected cells. Here we describe single-cell gene expression profiling of CA1 neurons in the rat hippocampus using a combination of laser capture, T7 RNA amplification, and cDNA microarray analysis. Subsequent cluster analysis of the microarray data identified two different cell types: pyramidal neurons and an interneuron. Cluster analysis also revealed differences among the ...[more]

PMID: 12736331

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Dataset Information

Single-cell microarray analysis in hippocampus CA1: demonstration and validation of cellular heterogeneity.

Publications

Single-cell microarray analysis in hippocampus CA1: demonstration and validation of cellular heterogeneity.

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