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C-terminal proteolysis of the collagen VI ?3 chain by BMP-1 and proprotein convertase(s) releases endotrophin in fragments of different sizes.


ABSTRACT: The assembly of collagen VI microfibrils is a multistep process in which proteolytic processing within the C-terminal globular region of the collagen VI ?3 chain plays a major role. However, the mechanisms involved remain elusive. Moreover, C5, the short and most C-terminal domain of the ?3 chain, recently has been proposed to be released as an adipokine that enhances tumor progression, fibrosis, inflammation, and insulin resistance and has been named "endotrophin." Serum endotrophin could be a useful biomarker to monitor the progression of such disorders as chronic obstructive pulmonary disease, systemic sclerosis, and kidney diseases. Here, using biochemical and isotopic MS-based analyses, we found that the extracellular metalloproteinase bone morphogenetic protein 1 (BMP-1) is involved in endotrophin release and determined the exact BMP-1 cleavage site. Moreover, we provide evidence that several endotrophin-containing fragments are present in various tissues and body fluids. Among these, a large C2-C5 fragment, which contained endotrophin, was released by furin-like proprotein convertase cleavage. By using immunofluorescence microscopy and EM, we also demonstrate that these proteolytic maturations occur after secretion of collagen VI tetramers and during microfibril assembly. Differential localization of N- and C-terminal regions of the collagen VI ?3 chain revealed that cleavage products are deposited in tissue and cell cultures. The detailed information on the processing of the collagen VI ?3 chain reported here provides a basis for unraveling the function of endotrophin (C5) and larger endotrophin-containing fragments and for refining their use as biomarkers of disease progression.

SUBMITTER: Heumuller SE 

PROVIDER: S-EPMC6746452 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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C-terminal proteolysis of the collagen VI α3 chain by BMP-1 and proprotein convertase(s) releases endotrophin in fragments of different sizes.

Heumüller Stefanie Elisabeth SE   Talantikite Maya M   Napoli Manon M   Armengaud Jean J   Mörgelin Matthias M   Hartmann Ursula U   Sengle Gerhard G   Paulsson Mats M   Moali Catherine C   Wagener Raimund R  

The Journal of biological chemistry 20190725 37


The assembly of collagen VI microfibrils is a multistep process in which proteolytic processing within the C-terminal globular region of the collagen VI α3 chain plays a major role. However, the mechanisms involved remain elusive. Moreover, C5, the short and most C-terminal domain of the α3 chain, recently has been proposed to be released as an adipokine that enhances tumor progression, fibrosis, inflammation, and insulin resistance and has been named "endotrophin." Serum endotrophin could be a  ...[more]

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