Ontology highlight
ABSTRACT:
SUBMITTER: Theil K
PROVIDER: S-EPMC6746756 | biostudies-literature | 2019 Sep
REPOSITORIES: biostudies-literature
Nature communications 20190916 1
Understanding regulation of an mRNA requires knowledge of its regulators. However, methods for reliable de-novo identification of proteins binding to a particular RNA are scarce and were thus far only successfully applied to abundant noncoding RNAs in cell culture. Here, we present vIPR, an RNA-protein crosslink, RNA pulldown, and shotgun proteomics approach to identify proteins bound to selected mRNAs in C. elegans. Applying vIPR to the germline-specific transcript gld-1 led to enrichment of kn ...[more]