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The RNA-mediated estrogen receptor ? interactome of hormone-dependent human breast cancer cell nuclei.


ABSTRACT: Estrogen Receptor alpha (ER?) is a ligand-inducible transcription factor that mediates estrogen signaling in hormone-responsive cells, where it controls key cellular functions by assembling in gene-regulatory multiprotein complexes. For this reason, interaction proteomics has been shown to represent a useful tool to investigate the molecular mechanisms underlying ER? action in target cells. RNAs have emerged as bridging molecules, involved in both assembly and activity of transcription regulatory protein complexes. By applying Tandem Affinity Purification (TAP) coupled to mass spectrometry (MS) before and after RNase digestion in vitro, we generated a dataset of nuclear ER? molecular partners whose association with the receptor involves RNAs. These data provide a useful resource to elucidate the combined role of nuclear RNAs and the proteins identified here in ER? signaling to the genome in breast cancer and other cell types.

SUBMITTER: Nassa G 

PROVIDER: S-EPMC6746822 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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The RNA-mediated estrogen receptor α interactome of hormone-dependent human breast cancer cell nuclei.

Nassa Giovanni G   Giurato Giorgio G   Salvati Annamaria A   Gigantino Valerio V   Pecoraro Giovanni G   Lamberti Jessica J   Rizzo Francesca F   Nyman Tuula A TA   Tarallo Roberta R   Weisz Alessandro A  

Scientific data 20190916 1


Estrogen Receptor alpha (ERα) is a ligand-inducible transcription factor that mediates estrogen signaling in hormone-responsive cells, where it controls key cellular functions by assembling in gene-regulatory multiprotein complexes. For this reason, interaction proteomics has been shown to represent a useful tool to investigate the molecular mechanisms underlying ERα action in target cells. RNAs have emerged as bridging molecules, involved in both assembly and activity of transcription regulator  ...[more]

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