Ontology highlight
ABSTRACT:
SUBMITTER: Dapkunas A
PROVIDER: S-EPMC6748995 | biostudies-literature | 2019 Sep
REPOSITORIES: biostudies-literature
Dapkunas Arvydas A Rantanen Ville V Gui Yujuan Y Lalowski Maciej M Sainio Kirsi K Kuure Satu S Sariola Hannu H
Scientific reports 20190917 1
Kidney mesenchyme (KM) and nephron progenitors (NPs) depend on WNT activity, and their culture in vitro requires extensive repertoire of recombinant proteins and chemicals. Here we established a robust, simple culture of mouse KM using a combination of 3D Matrigel and growth media supplemented with Fibroblast Growth Factor 2 (FGF2) and Src inhibitor PP2. This allows dissociated KM to spontaneously self-organize into spheres. To reassess the requirement of WNT activity in KM self-organization and ...[more]