Unknown

Dataset Information

0

Design of Sealable Custom-Shaped Cell Mimicries Based on Self-Assembled Monolayers on CYTOP Polymer.


ABSTRACT: In bottom-up synthetic biology, one of the major methodological challenges is to provide reaction spaces that mimic biological systems with regard to topology and surface functionality. Of particular interest are cell- or organelle-shaped membrane compartments, as many protein functions unfold at lipid interfaces. However, shaping artificial cell systems using materials with non-intrusive physicochemical properties, while maintaining flexible lipid interfaces relevant to the reconstituted protein systems, is not straightforward. Herein, we develop micropatterned chambers from CYTOP, a less commonly used polymer with good chemical resistance and a refractive index matching that of water. By forming a self-assembled lipid monolayer on the polymer surface, we dramatically increased the biocompatibility of CYTOP-fabricated systems. The phospholipid interface provides an excellent passivation layer to prevent protein adhesion to the hydrophobic surface, and we succeeded in cell-free protein synthesis inside the chambers. Importantly, the chambers could be sealed after loading by a lipid monolayer, providing a novel platform to study encapsulated systems. We successfully reconstituted pole-to-pole oscillations of the Escherichia coli MinDE system, which responds dramatically to compartment geometry. Furthermore, we present a simplified fabrication of our artificial cell compartments via replica molding, making it a readily accessible technique for standard cleanroom facilities.

SUBMITTER: Eto H 

PROVIDER: S-EPMC6750829 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

altmetric image

Publications

Design of Sealable Custom-Shaped Cell Mimicries Based on Self-Assembled Monolayers on CYTOP Polymer.

Eto Hiromune H   Soga Naoki N   Franquelim Henri G HG   Glock Philipp P   Khmelinskaia Alena A   Kai Lei L   Heymann Michael M   Noji Hiroyuki H   Schwille Petra P  

ACS applied materials & interfaces 20190607 24


In bottom-up synthetic biology, one of the major methodological challenges is to provide reaction spaces that mimic biological systems with regard to topology and surface functionality. Of particular interest are cell- or organelle-shaped membrane compartments, as many protein functions unfold at lipid interfaces. However, shaping artificial cell systems using materials with non-intrusive physicochemical properties, while maintaining flexible lipid interfaces relevant to the reconstituted protei  ...[more]

Similar Datasets

| S-EPMC3458608 | biostudies-literature
| S-EPMC3549517 | biostudies-literature
| S-EPMC7504895 | biostudies-literature
| S-EPMC6271350 | biostudies-literature
| S-EPMC8262134 | biostudies-literature
| S-EPMC6941449 | biostudies-literature
| S-EPMC5302704 | biostudies-other
| S-EPMC2787851 | biostudies-literature
| S-EPMC9501787 | biostudies-literature
| S-EPMC3011841 | biostudies-other