Unknown

Dataset Information

0

"Water-like" Dual-Functionalized Ionic Liquids for Enzyme Activation.


ABSTRACT: By mimicking the water structure to improve the enzyme activity, we designed imidazolium (Im)-based ionic liquids (ILs) functionalized with both ether and tert-alcohol groups (e.g., [CH3(OCH2CH2) n -Im-t-BuOH][Tf2N]). This unique combination of the "water-like" structure enabled very high transesterification (synthetic) activities for immobilized lipase B from Candida antarctica, which are up to 2-4 folds higher than nonfunctionalized "classical" ionic liquids (such as [BMIM][Tf2N]) and up to 40-100% higher than diisopropyl ether and tert-butanol. Fluorescence emission spectra confirmed the general protein structural preservation in these tailored ionic solvents. In addition, functionalized ILs showed high thermal stabilities, which are comparable with diisopropyl ether but much higher than tert-butanol.

SUBMITTER: Zhao H 

PROVIDER: S-EPMC6751713 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

altmetric image

Publications

"Water-like" Dual-Functionalized Ionic Liquids for Enzyme Activation.

Zhao Hua H   Harter Glenn A GA   Martin Caden J CJ  

ACS omega 20190904 12


By mimicking the water structure to improve the enzyme activity, we designed imidazolium (Im)-based ionic liquids (ILs) functionalized with both ether and <i>tert</i>-alcohol groups (e.g., [CH<sub>3</sub>(OCH<sub>2</sub>CH<sub>2</sub>) <i><sub>n</sub></i> -Im-<i>t</i>-BuOH][Tf<sub>2</sub>N]). This unique combination of the "water-like" structure enabled very high transesterification (synthetic) activities for immobilized lipase B from <i>Candida antarctica</i>, which are up to 2-4 folds higher t  ...[more]

Similar Datasets

| S-EPMC4726069 | biostudies-literature
| S-EPMC7796259 | biostudies-literature
| S-EPMC4448549 | biostudies-other
| S-EPMC8658901 | biostudies-literature
| S-EPMC7181116 | biostudies-literature
| S-EPMC6804084 | biostudies-literature
| S-EPMC6175172 | biostudies-literature
| S-EPMC9779220 | biostudies-literature
| S-EPMC8243323 | biostudies-literature
2021-08-13 | GSE156769 | GEO