Ontology highlight
ABSTRACT:
SUBMITTER: Chang EY
PROVIDER: S-EPMC6753070 | biostudies-literature | 2019 Sep
REPOSITORIES: biostudies-literature
Nature communications 20190919 1
Ectopic R-loop accumulation causes DNA replication stress and genome instability. To avoid these outcomes, cells possess a range of anti-R-loop mechanisms, including RNaseH that degrades the RNA moiety in R-loops. To comprehensively identify anti-R-loop mechanisms, we performed a genome-wide trigenic interaction screen in yeast lacking RNH1 and RNH201. We identified >100 genes critical for fitness in the absence of RNaseH, which were enriched for DNA replication fork maintenance factors includin ...[more]