A phosphorylation-deficient mutant of retinoid X receptor ? at Thr 167 alters fasting response and energy metabolism in mice.

ABSTRACT: Retinoid X receptor ? (RXR?) has a conserved phosphorylation motif at threonine 162 (humans) and threonine 167 (mice) within the DNA-binding domain. Here we have generated RXR? knock-in mice (Rxr?T167A) bearing a single mutation of Thr 167 to alanine and examined the roles of Thr 167 in the regulation of energy metabolism within adipose, muscle, and liver tissues. Rxr?T167A mice exhibited down-regulation of metabolic pathways converting glucose to fatty acids, such as acetyl-CoA carboxylase in the white adipose tissue (WAT) and ATP citrate lyase in the muscle. They also reduced gene expression for genes related to fatty acid catabolism and triglyceride synthesis in WAT and controlled heat factors such as adrenergic receptor ?1 in muscles. In contrast, hepatic gluconeogenic pathways and synthetic pathways related to fatty acids remained unaffected by this mutation. Expression of multiple genes that were affected by the Thr 167 mutation in adipose tissue exhibited clear response to LG100268, a synthetic RXR agonist. Thus, the altered gene expression in mutant mice adipose appeared to be a direct effect of RXR? Thr 167 mutation and by some secondary effect of the mutation. Blood glucose levels remained normal in Rxr?T167A during feeding, as observed with RXR? wild-type mice. However, Rxr?T167A mice exhibited an attenuated decrease of blood glucose levels that occurred after fasting. This attenuation correlated with a concomitant down-regulation of lipid metabolism in WAT and was associated with RXR? phosphorylation at Thr 167. Thus, Thr 167 enabled RXR? to coordinate these three organs for regulation of energy metabolism and maintenance of glucose homeostasis.

SUBMITTER: Sueyoshi T 

PROVIDER: S-EPMC6759383 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature