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Activation of Piezo1 mechanosensitive ion channel in HEK293T cells by 30?MHz vertically deployed surface acoustic waves.

ABSTRACT: Ultrasound (US) has emerged as a promising noninvasive modality for neuromodulation. Despite previous evidence that US may mediate cellular response by activating mechanosensitive ion channels embedded in the cell membrane, the underlying mechanism is not well understood. In this work, we developed a vertically deployed surface acoustic wave (VD-SAW) platform that generates 30?MHz focused ultrasound wave for mechanical stimulation of single cells. We investigated the role of Piezo1 in mediating the intracellular calcium response ( [Formula: see text] ) of HEK293T cells in response to pulsed US operated at a peak pressure of 1.6?MPa with 20% duty cycle, and a total treatment time of 60?s. We observed that the elicited calcium response depends critically on the pulse repetition frequency (PRF) or burst duration of the US, as well as the presence of the Piezo1. Significantly higher [Formula: see text] increase was produced in the Piezo1-transfected (P1TF) than in the Piezo1-knockout (P1KO) HEK293T cells. Furthermore, higher calcium response probability, stronger and faster [Formula: see text] increase, and greater cell displacement were produced at 2?Hz PRF with 100?ms burst duration than 200?Hz PRF with 1?ms burst duration. Altogether, we have demonstrated that the VD-SAW platform provides a unique and versatile tool for investigating US-induced mechanotransduction at the single cell level.

SUBMITTER: Liao D

PROVIDER: S-EPMC6761923 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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Activation of Piezo1 mechanosensitive ion channel in HEK293T cells by 30 MHz vertically deployed surface acoustic waves.

Liao Defei D Li Fenfang F Lu David D Zhong Pei P

Biochemical and biophysical research communications 20190823 3

Ultrasound (US) has emerged as a promising noninvasive modality for neuromodulation. Despite previous evidence that US may mediate cellular response by activating mechanosensitive ion channels embedded in the cell membrane, the underlying mechanism is not well understood. In this work, we developed a vertically deployed surface acoustic wave (VD-SAW) platform that generates 30 MHz focused ultrasound wave for mechanical stimulation of single cells. We investigated the role of Piezo1 in mediating ...[more]

PMID: 31451220

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Project description:The space plasmas have been found empirically to be separated into those residing far from the classical thermal equilibrium and those residing near equilibrium. The modern formalism of the kappa distributions explains this distinction under the value of the kappa index, the intensive parameter that characterizes thermodynamics together with temperature. Recent studies have suggested that by defining an invariant kappa index as the zero dimensionality spectral index, [Formula: see text], which is independent of the dimensionality, the degrees of freedom, or the numbers of particles, one may separately consider the physical and thermodynamic feature of the kappa index in space plasmas by utilizing [Formula: see text]. This study extends the mentioned idea to the ion-acoustic waves (IAWs) in the astrophysical plasmas in order to deriving an invariant formalism for the IAWs including the pure thermodynamic features of the background particles. This paper is based on the kinetic theory formalism and the hydrodynamic fluid description for extracting the characteristics of the invariant IAWs. Relying on the Vlasov-Poisson equations, considering a low-frequency band for the weakly damped ion oscillations, we have derived the most generalized formalism of the ion-sound speed in space plasmas in terms of the extended polytropic indices of the plasma species, [Formula: see text], and also the generalized formalism of Landau damping for the invariant IAWs in terms of [Formula: see text], wavelength, and temperatures of the plasma species. In the hydrodynamic description, we have normalized the fluid parameters in terms of the generalized quantities, including the extended formulations of the ion-sound speed and Debye length. Then, by using the perturbation expansion in linear and nonlinear regimes, we may find some other issues in the formalism of the invariant IAWs, such as the effect of the perturbed potential degrees of freedom, [Formula: see text], the isothermal/extended phase speed of the IAWs, and the combined effects of the wave steepening and dispersion of ion waves. We have also derived a generalized KdV equation and its solitary wave solutions in an invariant formalism. Based on the empirical evidences in space plasmas, the far-equilibrium plasmas are characterized by [Formula: see text] ([Formula: see text]), while the near-equilibrium plasmas are labeled with [Formula: see text] ([Formula: see text]). We have numerically analyzed our solutions from the anti-equilibrium states at [Formula: see text] ([Formula: see text]) towards the equilibrium states at [Formula: see text] ([Formula: see text]). Our theoretical study provides strong evidence, for the first time, about the distinction of plasmas under the value of the kappa index. Our analysis confirms the distinction of the involved IAWs diagrams in the two mentioned regions, where the transition from far-equilibrium states to the near-equilibrium states may occur in the vicinity [Formula: see text] ([Formula: see text]), denoting the escape state of the evolution.

Dataset Information

Activation of Piezo1 mechanosensitive ion channel in HEK293T cells by 30?MHz vertically deployed surface acoustic waves.

Publications

Activation of Piezo1 mechanosensitive ion channel in HEK293T cells by 30 MHz vertically deployed surface acoustic waves.

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Activation of Piezo1 mechanosensitive ion channel in HEK293T cells by 30?MHz vertically deployed surface acoustic waves.

Publications

Activation of Piezo1 mechanosensitive ion channel in HEK293T cells by 30 MHz vertically deployed surface acoustic waves.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

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Activation of Piezo1 mechanosensitive ion channel in HEK293T cells by 30 MHz vertically deployed surface acoustic waves.