Next-Generation Sequencing for the Diagnosis of Challenging Culture-Negative Endocarditis.
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ABSTRACT:
SUBMITTER: Kolb M
PROVIDER: S-EPMC6763761 | biostudies-literature | 2019
REPOSITORIES: biostudies-literature
Publications
Next-Generation Sequencing for the Diagnosis of Challenging Culture-Negative Endocarditis.
Kolb Manon M Lazarevic Vladimir V Emonet Stéphane S Calmy Alexandra A Girard Myriam M Gaïa Nadia N Charretier Yannick Y Cherkaoui Abdessalam A Keller Peter P Huber Christoph C Schrenzel Jacques J
Frontiers in medicine 20190920
Diagnosis of culture-negative infective endocarditis usually implies indirect pathogen identification by serologic or molecular techniques. Clinical metagenomics, relying on next-generation sequencing (NGS) is an emerging approach that allows pathogen identification in challenging situations, as evidenced by a clinical case. We sequenced the DNA extracted from the surgically-removed frozen valve tissue from a patient with suspected infective endocarditis with negative blood and valve cultures. M ...[more]
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