Ontology highlight
ABSTRACT:
SUBMITTER: Khavari A
PROVIDER: S-EPMC6771309 | biostudies-literature | 2019
REPOSITORIES: biostudies-literature
Khavari Adele A Ehrlicher Allen Joseph AJ
PloS one 20190912 9
Measuring pressures within complex multi-cellular environments is critical for studying mechanobiology as these forces trigger diverse biological responses, however, these studies are difficult as a deeply embedded yet well-calibrated probe is required. In this manuscript, we use endogenous cell nuclei as pressure sensors by introducing a fluorescent protein localized to the nucleus and confocal microscopy to measure the individual nuclear volumes in 3D multi-cellular aggregates. We calibrate th ...[more]