Dataset Information

Soil HONO emissions at high moisture content are driven by microbial nitrate reduction to nitrite: tackling the HONO puzzle.

ABSTRACT: Nitrous acid (HONO) is a precursor of the hydroxyl radical (OH), a key oxidant in the degradation of most air pollutants. Field measurements indicate a large unknown source of HONO during the day time. Release of nitrous acid (HONO) from soil has been suggested as a major source of atmospheric HONO. We hypothesize that nitrite produced by biological nitrate reduction in oxygen-limited microzones in wet soils is a source of such HONO. Indeed, we found that various contrasting soil samples emitted HONO at high water-holding capacity (75-140%), demonstrating this to be a widespread phenomenon. Supplemental nitrate stimulated HONO emissions, whereas ethanol (70% v/v) treatment to minimize microbial activities reduced HONO emissions by 80%, suggesting that nitrate-dependent biotic processes are the sources of HONO. High-throughput Illumina sequencing of 16S rRNA as well as functional gene transcripts associated with nitrate and nitrite reduction indicated that HONO emissions from soil samples were associated with nitrate reduction activities of diverse Proteobacteria. Incubation of pure cultures of bacterial nitrate reducers and gene-expression analyses, as well as the analyses of mutant strains deficient in nitrite reductases, showed positive correlations of HONO emissions with the capability of microbes to reduce nitrate to nitrite. Thus, we suggest biological nitrate reduction in oxygen-limited microzones as a hitherto unknown source of atmospheric HONO, affecting biogeochemical nitrogen cycling, atmospheric chemistry, and global modeling.

SUBMITTER: Wu D

PROVIDER: S-EPMC6776056 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Soil HONO emissions at high moisture content are driven by microbial nitrate reduction to nitrite: tackling the HONO puzzle.

Wu Dianming D Horn Marcus A MA Behrendt Thomas T Müller Stefan S Li Jingsong J Cole Jeff A JA Xie Baohua B Ju Xiaotang X Li Guo G Ermel Michael M Oswald Robert R Fröhlich-Nowoisky Janine J Hoor Peter P Hu Chunsheng C Liu Min M Andreae Meinrat O MO Pöschl Ulrich U Cheng Yafang Y Su Hang H Trebs Ivonne I Weber Bettina B Sörgel Matthias M

The ISME journal 20190304 7

Nitrous acid (HONO) is a precursor of the hydroxyl radical (OH), a key oxidant in the degradation of most air pollutants. Field measurements indicate a large unknown source of HONO during the day time. Release of nitrous acid (HONO) from soil has been suggested as a major source of atmospheric HONO. We hypothesize that nitrite produced by biological nitrate reduction in oxygen-limited microzones in wet soils is a source of such HONO. Indeed, we found that various contrasting soil samples emitted ...[more]

PMID: 30833686

Similar Datasets

Project description:Nitrate reductases (NRs) are molybdoenzymes that reduce nitrate (NO3 -) to nitrite (NO2 -) in both mammals and plants. In mammals, the salival microbes take part in the generation of the NO2 - from NO3 -, which further produces nitric oxide (NO) either in acid-induced NO2 - reduction or in the presence of nitrite reductases (NiRs). Here, we report a new approach of VCl3 (V3+ ion source) induced step-wise reduction of NO3 - in a CoII-nitrato complex, [(12-TMC)CoII(NO3 -)]+ (2,{CoII-NO3 -}), to a CoIII-nitrosyl complex, [(12-TMC)CoIII(NO)]2+ (4,{CoNO}8), bearing an N-tetramethylated cyclam (TMC) ligand. The VCl3 inspired reduction of NO3 - to NO is believed to occur in two consecutive oxygen atom transfer (OAT) reactions, i.e., OAT-1 = NO3 - → NO2 - (r1) and OAT-2 = NO2 - → NO (r2). In these OAT reactions, VCl3 functions as an O-atom abstracting species, and the reaction of 2 with VCl3 produces a CoIII-nitrosyl ({CoNO}8) with VV-Oxo ({VV[double bond, length as m-dash]O}3+) species, via a proposed CoII-nitrito (3, {CoII-NO2 -}) intermediate species. Further, in a separate experiment, we explored the reaction of isolated complex 3 with VCl3, which showed the generation of 4 with VV-Oxo, validating our proposed reaction sequences of OAT reactions. We ensured and characterized 3 using VCl3 as a limiting reagent, as the second-order rate constant of OAT-2 (k 2 /) is found to be ∼1420 times faster than that of the OAT-1 (k 2) reaction. Binding constant (K b) calculations also support our proposition of NO3 - to NO transformation in two successive OAT reactions, as K b(CoII-NO2 -) is higher than K b(CoII-NO3 -), hence the reaction moves in the forward direction (OAT-1). However, K b(CoII-NO2 -) is comparable to K b{CoNO}8 , and therefore sequenced the second OAT reaction (OAT-2). Mechanistic investigations of these reactions using 15N-labeled-15NO3 - and 15NO2 - revealed that the N-atom in the {CoNO}8 is derived from NO3 - ligand. This work highlights the first-ever report of VCl3 induced step-wise NO3 - reduction (NRs activity) followed by the OAT induced NO2 - reduction and then the generation of Co-nitrosyl species {CoNO}8.

Project description:The rheological properties parameters of paddy soil affect the interaction between the tillage tools and soil, thus influencing the operation quality and power consumption. In order to study the effects of tillage methods and moisture content on the rheological properties parameters of paddy soil in the middle and lower reaches of the Yangtze River, uniaxial compression creep tests of paddy soils with four moisture contents under no tillage (moisture contents: 26.71%, 24.52%, 23.26%, 21.28%) and plough tillage (moisture contents: 26.77%, 25.55%, 23.40%, 20.56%) were carried out using a TMS-PRO texture analyzer. The creep properties curves obtained from the tests, and the rheological constitutive equation of paddy soil under compression was established by Burgers viscoelastic model. Respectively, the quantitative change rules of creep properties of paddy soil with different moisture contents under different tillage methods and the correlation between these parameters were explored. The results showed that the moisture content under the three-year plough tillage and no tillage methods had significant influence on the rheological properties parameters of paddy soil (P < 0.05). The instantaneous elastic modulus, delay elastic modulus, and viscosity coefficient of the two paddy soils (no tillage and plough tillage soils) decreased with the increase of moisture content. However, the variation rules of relaxation time and delay viscosity coefficient with moisture content differed between these two paddy soils. Specifically, the strain rate of the two paddy soils decreased as moisture content decreased, where the total strain combines elastic strain, viscous strain, and viscoelastic strain. The initial strain rate and steady strain rate of the plough tillage paddy soils were lower than that of the no tillage paddy soils. The established creep model equation could be used to obtain viscoelastic rheological parameters of paddy soil in a wide range. The fitting equations between rheological parameters and moisture content were introduced into Burgers model, and the coupling equations between creep deformation and moisture content and time were derived, which could be used to predict the creep properties and deformation behavior of paddy soil in a certain range of no tillage and ploughed field. Overall, this study has a certain theoretical significance for the development and improvement of paddy soil rheology theory, and can also provide theoretical basis and technical support for the research of agricultural machinery design optimization, field water, soil conservation, soil tillage and compaction related simulation analysis in the middle and lower reaches of the Yangtze River.

Dataset Information

Soil HONO emissions at high moisture content are driven by microbial nitrate reduction to nitrite: tackling the HONO puzzle.

Publications

Soil HONO emissions at high moisture content are driven by microbial nitrate reduction to nitrite: tackling the HONO puzzle.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets