Ontology highlight
ABSTRACT:
SUBMITTER: Ebrahimi SB
PROVIDER: S-EPMC6777339 | biostudies-literature | 2019 Sep
REPOSITORIES: biostudies-literature
Ebrahimi Sasha B SB Samanta Devleena D Cheng Ho Fung HF Nathan Levy I LI Mirkin Chad A CA
Journal of the American Chemical Society 20190823 35
Aptamers are oligonucleotide sequences that can be evolved to bind to various analytes of interest. Here, we present a general design strategy that transduces an aptamer-target binding event into a fluorescence readout via the use of a viscosity-sensitive dye. Target binding to the aptamer leads to forced intercalation (FIT) of the dye between oligonucleotide base pairs, increasing its fluorescence by up to 20-fold. Specifically, we demonstrate that FIT-aptamers can report target presence throug ...[more]