Ontology highlight
ABSTRACT:
SUBMITTER: Gibson BA
PROVIDER: S-EPMC6778041 | biostudies-literature | 2019 Oct
REPOSITORIES: biostudies-literature
Gibson Bryan A BA Doolittle Lynda K LK Schneider Maximillian W G MWG Jensen Liv E LE Gamarra Nathan N Henry Lisa L Gerlich Daniel W DW Redding Sy S Rosen Michael K MK
Cell 20190919 2
Eukaryotic chromatin is highly condensed but dynamically accessible to regulation and organized into subdomains. We demonstrate that reconstituted chromatin undergoes histone tail-driven liquid-liquid phase separation (LLPS) in physiologic salt and when microinjected into cell nuclei, producing dense and dynamic droplets. Linker histone H1 and internucleosome linker lengths shared across eukaryotes promote phase separation of chromatin, tune droplet properties, and coordinate to form condensates ...[more]