Unknown

Dataset Information

0

Hyperosmotic stress: in situ chromatin phase separation.


ABSTRACT: Dehydration of cells by acute hyperosmotic stress has profound effects upon cell structure and function. Interphase chromatin and mitotic chromosomes collapse ("congelation"). HL-60/S4 cells remain ~100% viable for, at least, 1 hour, exhibiting shrinkage to ~2/3 their original volume, when placed in 300mM sucrose in tissue culture medium. Fixed cells were imaged by immunostaining confocal and STED microscopy. At a "global" structural level (μm), mitotic chromosomes congeal into a residual gel with apparent (phase) separations of Ki67, CTCF, SMC2, RAD21, H1 histones and HMG proteins. At an "intermediate" level (sub-μm), radial distribution analysis of STED images revealed a most probable peak DNA density separation of ~0.16 μm, essentially unchanged by hyperosmotic stress. At a "local" structural level (~1-2 nm), in vivo crosslinking revealed essentially unchanged crosslinked products between H1, HMG and inner histones. Hyperosmotic cellular stress is discussed in terms of concepts of mitotic chromosome structure and liquid-liquid phase separation.

SUBMITTER: Olins AL 

PROVIDER: S-EPMC6973338 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC6833219 | biostudies-literature
| S-EPMC8139833 | biostudies-literature
| S-EPMC10723116 | biostudies-literature
| S-EPMC8647409 | biostudies-literature
| S-EPMC6778041 | biostudies-literature
| S-EPMC10907630 | biostudies-literature
| S-EPMC10769318 | biostudies-literature
| S-EPMC5311949 | biostudies-literature
| S-EPMC8604804 | biostudies-literature
| S-EPMC10065442 | biostudies-literature