Dataset Information

Dendronized fluorosurfactant for highly stable water-in-fluorinated oil emulsions with minimal inter-droplet transfer of small molecules.

ABSTRACT: Fluorosurfactant-stabilized microfluidic droplets are widely used as pico- to nanoliter volume reactors in chemistry and biology. However, current surfactants cannot completely prevent inter-droplet transfer of small organic molecules encapsulated or produced inside the droplets. In addition, the microdroplets typically coalesce at temperatures higher than 80?°C. Therefore, the use of droplet-based platforms for ultrahigh-throughput combination drug screening and polymerase chain reaction (PCR)-based rare mutation detection has been limited. Here, we provide insights into designing surfactants that form robust microdroplets with improved stability and resistance to inter-droplet transfer. We used a panel of dendritic oligo-glycerol-based surfactants to demonstrate that a high degree of inter- and intramolecular hydrogen bonding, as well as the dendritic architecture, contribute to high droplet stability in PCR thermal cycling and minimize inter-droplet transfer of the water-soluble fluorescent dye sodium fluorescein salt and the drug doxycycline.

SUBMITTER: Chowdhury MS

PROVIDER: S-EPMC6778136 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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Dendronized fluorosurfactant for highly stable water-in-fluorinated oil emulsions with minimal inter-droplet transfer of small molecules.

Chowdhury Mohammad Suman MS Zheng Wenshan W Kumari Shalini S Heyman John J Zhang Xingcai X Dey Pradip P Weitz David A DA Haag Rainer R

Nature communications 20191004 1

Fluorosurfactant-stabilized microfluidic droplets are widely used as pico- to nanoliter volume reactors in chemistry and biology. However, current surfactants cannot completely prevent inter-droplet transfer of small organic molecules encapsulated or produced inside the droplets. In addition, the microdroplets typically coalesce at temperatures higher than 80 °C. Therefore, the use of droplet-based platforms for ultrahigh-throughput combination drug screening and polymerase chain reaction (PCR)- ...[more]

PMID: 31586046

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Project description:Vortex-based hydrodynamic cavitation offers an effective platform for producing emulsions. In this work, we have investigated characteristics of dense oil in water emulsions with oil volume fractions up to 60% produced using a vortex-based cavitation device. Emulsions were prepared using rapeseed oil with oil volume fractions of 0.15, 0.3, 0.45, and 0.6. For each of these volume fractions, the pressure drop as a function of the flow rate of emulsions through the cavitation device was measured. These data were used for estimating the effective viscosity of the emulsions. The droplet size distribution of the emulsions was measured using the laser diffraction technique. The influence of the number of passes through the cavitation device on droplet size distributions and the Sauter mean diameter was quantified. It was found that the Sauter mean diameter (d32) decreases with an increase in the number of passes as n-0.2. The Sauter mean diameter was found to be almost independent of oil volume fraction (αo) up to a certain critical volume fraction (αoc). Beyond αoc, d32 was found to be linearly proportional to a further increase in oil volume fraction. As expected, the turbidity of the produced emulsions was found to be linearly proportional to the oil volume fraction. The slope of turbidity versus oil volume fraction can be used to estimate the Sauter mean diameter. A suitable correlation was developed to relate turbidity, volume fraction, and Sauter mean diameter. The droplet breakage efficiency of the vortex-based cavitation device for dense oil in water emulsions was quantified and reported. The breakage efficiency was found to increase linearly with an increase in oil volume fraction up to αoc and then plateau with a further increase in the oil volume fraction. The breakage efficiency was found to decrease with an increase in energy consumption per unit mass (E) as E-0.8. The presented results demonstrate the effectiveness of a vortex-based cavitation device for producing dense oil in water emulsions and will be useful for extending its applications to other dense emulsions.

Dataset Information

Dendronized fluorosurfactant for highly stable water-in-fluorinated oil emulsions with minimal inter-droplet transfer of small molecules.

Publications

Dendronized fluorosurfactant for highly stable water-in-fluorinated oil emulsions with minimal inter-droplet transfer of small molecules.

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