Dataset Information

Evaluation of Antibacterial Effect of Propolis and its Application in Mouthwash Production.

ABSTRACT: Objectives:Our purpose was to determine the antibacterial properties of propolis and to evaluate its use as an antibacterial mouthwash with minimal complications. Materials and Methods:In this experimental laboratory study, an alcoholic propolis extract was prepared. The minimum inhibitory concentration (MIC) was calculated for four bacterial species including Staphylococcus aureus (S. aureus), Streptococcus mutans (S. mutans), Lactobacillus acidophilus (L. acidophilus), and Enterococcus faecalis (E. faecalis) using agar dilution. According to the MIC, a propolis antibacterial mouthwash was produced and compared to water, chlorhexidine (CHX), and Listerine using laboratory rats for clinical examination. Salivary specimens of rats were collected at 12 hours, 1 week, and 2 weeks after using the mouthwash and examined by real-time polymerase chain reaction (RT-PCR). Data were analyzed using one-way analysis of variance (ANOVA) and repeated measures ANOVA (?=0.05). Results:The results of agar dilution by the number of colony-forming units showed the lowest MIC for S. aureus and the highest for L. acidophilus. Our RT-PCR findings indicated that water alone had no effect on the level of oral bacteria. Propolis mouthwash showed a significant difference with CHX and Listerine (P<0.05) in terms of the number of S. mutans, E. faecalis, and L. acidophilus colonies, while CHX and Listerine were less efficient. There was no significant difference between CHX and propolis (P=0.110) regarding S. aureus colonies, but Listerine had a lower efficacy than either (P<0.05). Conclusion:According to the results, propolis mouthwash was more efficient against the studied oral bacteria compared to CHX and Listerine.

SUBMITTER: Nazeri R

PROVIDER: S-EPMC6778618 | biostudies-literature | 2019 Jan-Feb

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Evaluation of Antibacterial Effect of Propolis and its Application in Mouthwash Production.

Nazeri Rahman R Ghaiour Marzieh M Abbasi Shima S

Frontiers in dentistry 20190120 1

<h4>Objectives</h4>Our purpose was to determine the antibacterial properties of propolis and to evaluate its use as an antibacterial mouthwash with minimal complications.<h4>Materials and methods</h4>In this experimental laboratory study, an alcoholic propolis extract was prepared. The minimum inhibitory concentration (MIC) was calculated for four bacterial species including <i>Staphylococcus aureus</i> (<i>S. aureus</i>), <i>Streptococcus mutans</i> (<i>S. mutans</i>), <i>Lactobacillus acidophi ...[more]

PMID: 31608331

Similar Datasets

Project description:BackgroundPropolis (bee glue) is a resinous honeybee product having a long history of application in many countries as a traditional remedy for treating wounds, burns, soar throat, stomach disorders, etc. It has been proved to possess beneficial biological effects, including antimicrobial, antioxidant, anti-inflammatory, cytotoxic, antiulcer, and many others. Bees gather propolis from diverse resinous plant parts and in different phytogeographic regions its chemical composition might vary significantly. In this article we report the results of the first study on the chemical profiles of propolis from Oman, its plant origin and antibacterial activity.ResultsThe chemical profiles of Omani propolis extracts were obtained by GC-MS analysis after silylation. Over 50 individual compounds were identified in the samples, belonging to different compound types: sugars, polyols, hydroxy acids, fatty acids, cardanols and cardols, anacardic acids, flavan derivatives, triterpenes, prenylated flavanones and chalcones. The profiles were dissimilar from other known propolis types. They demonstrate that although Oman is not a large country, the plant sources of propolis vary significantly, even in the same apiary and the same season. Based on chemical profiles, and isolation and identification of major marker compounds (new propolis constituents), new plant sources of propolis were found: Azadiracta indica (neem tree) and Acacia spp. (most probably A. nilotica). The ethanol extracts of the studied propolis samples demonstrated activity against S. aureus (MIC?<?100 ?g. mL-1) and E. coli (MIC?<?380 ?g. mL-1).ConclusionOmani propolis is different form the known propolis types and demonstrates significant chemical diversity. Its most important plant source is the resin of Azadirachta indica, and as a result its typical components are ?5-prenyl flavanones. Other plant sources have been identified, too, playing some role in resin collection by bees in Oman: Acacia spp. (most probably A. nilotica) and Mangifera indica. The results demonstrate also the potential of Omani propolis as antimicrobial.

Dataset Information

Evaluation of Antibacterial Effect of Propolis and its Application in Mouthwash Production.

Publications

Evaluation of Antibacterial Effect of Propolis and its Application in Mouthwash Production.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets