Unknown

Dataset Information

0

HER2 Amplification in Tumors Activates PI3K/Akt Signaling Independent of HER3.


ABSTRACT: Current evidence suggests that HER2-driven tumorigenesis requires HER3. This is likely due to the unique ability of HER3 to activate PI3K/Akt pathway signaling, which is not directly accessible to HER2. By genetic elimination of HER3 or shRNA knockdown of HER3 in HER2-amplified cancer cells, we find residual HER2-driven activation of PI3K/Akt pathway signaling that is driven by HER2 through direct and indirect mechanisms. Indirect mechanisms involved second messenger pathways, including Ras or Grb2. Direct binding of HER2 to PI3K occurred through p-Tyr1139, which has a weak affinity for PI3K but becomes significant at very high expression and phosphorylation. Mutation of Y1139 impaired the tumorigenic competency of HER2. Total elimination of HER3 expression in HCC1569 HER2-amplified cancer cells significantly impaired tumorigenicity only transiently, overcome by subsequent increases in HER2 expression and phosphorylation with binding and activation of PI3K. In contrast to activation of oncogenes by mutation, activation by overexpression was quantitative in nature: weak intrinsic activities were strengthened by overexpression, with additional gains observed through further increases in expression. Collectively, these data show that progressive functional gains by HER2 can increase its repertoire of activities such as the activation of PI3K and overcome its dependency on HER3.Significance: The intrinsic ability of HER2 to activate PI3K correlates with increased HER2 expression and can supplant the dependency upon HER3 for growth in HER2-amplified cancers. Cancer Res; 78(13); 3645-58. ©2018 AACR.

SUBMITTER: Ruiz-Saenz A 

PROVIDER: S-EPMC6779043 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature

altmetric image

Publications

HER2 Amplification in Tumors Activates PI3K/Akt Signaling Independent of HER3.

Ruiz-Saenz Ana A   Dreyer Courtney C   Campbell Marcia R MR   Steri Veronica V   Gulizia Nate N   Moasser Mark M MM  

Cancer research 20180514 13


Current evidence suggests that HER2-driven tumorigenesis requires HER3. This is likely due to the unique ability of HER3 to activate PI3K/Akt pathway signaling, which is not directly accessible to HER2. By genetic elimination of HER3 or shRNA knockdown of HER3 in HER2-amplified cancer cells, we find residual HER2-driven activation of PI3K/Akt pathway signaling that is driven by HER2 through direct and indirect mechanisms. Indirect mechanisms involved second messenger pathways, including Ras or G  ...[more]

Similar Datasets

| S-EPMC2516933 | biostudies-literature
| S-EPMC3790862 | biostudies-literature
| S-EPMC5334195 | biostudies-literature
| S-EPMC6597087 | biostudies-literature
| S-EPMC3979058 | biostudies-literature
| S-EPMC3638576 | biostudies-literature
| S-EPMC3916952 | biostudies-literature
| S-EPMC4674361 | biostudies-literature
| S-EPMC5494680 | biostudies-other
| S-ECPF-GEOD-42781 | biostudies-other