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Method comparison for N-glycan profiling: Towards the standardization of glycoanalytical technologies for cell line analysis.

ABSTRACT: The study of protein N-glycosylation is essential in biological and biopharmaceutical research as N-glycans have been reported to regulate a wide range of physiological and pathological processes. Monitoring glycosylation in diagnosis, prognosis, as well as biopharmaceutical development and quality control are important research areas. A number of techniques for the analysis of protein N-glycosylation are currently available. Here we examine three methodologies routinely used for the release of N-glycans, in the effort to establish and standardize glycoproteomics technologies for quantitative glycan analysis from cultured cell lines. N-glycans from human gamma immunoglobulins (IgG), plasma and a pool of four cancer cell lines were released following three approaches and the performance of each method was evaluated.

SUBMITTER: Kotsias M

PROVIDER: S-EPMC6779296 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Method comparison for N-glycan profiling: Towards the standardization of glycoanalytical technologies for cell line analysis.

Kotsias Maximilianos M Blanas Athanasios A van Vliet Sandra J SJ Pirro Martina M Spencer Daniel I R DIR Kozak Radoslaw P RP

PloS one 20191007 10

The study of protein N-glycosylation is essential in biological and biopharmaceutical research as N-glycans have been reported to regulate a wide range of physiological and pathological processes. Monitoring glycosylation in diagnosis, prognosis, as well as biopharmaceutical development and quality control are important research areas. A number of techniques for the analysis of protein N-glycosylation are currently available. Here we examine three methodologies routinely used for the release of ...[more]

PMID: 31589631

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