Simultaneous detection of multiple DNA damage types by multi-colour fluorescent labelling.
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ABSTRACT:
SUBMITTER: Torchinsky D
PROVIDER: S-EPMC6783632 | biostudies-literature | 2019 Sep
REPOSITORIES: biostudies-literature
Publications
Simultaneous detection of multiple DNA damage types by multi-colour fluorescent labelling.
Torchinsky Dmitry D Michaeli Yael Y Gassman Natalie R NR Ebenstein Yuval Y
Chemical communications (Cambridge, England) 20190901 76
Herein we present an assay allowing concurrent detection of oxidative DNA damage and photoproducts. We apply DNA repair enzymes specific for each lesion type to incorporate spectrally distinct fluorescent nucleotides, enabling simultaneous quantification of the lesions on individual DNA molecules. We follow the repair of both damage types in skin cells exposed to artificial sunlight. ...[more]
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