Dataset Information

Cucurbit Chlorotic Yellows Virus p22 Protein Interacts with Cucumber SKP1LB1 and Its F-Box-Like Motif Is Crucial for Silencing Suppressor Activity.

ABSTRACT: Plants use RNA silencing as a defense against viruses. In response, viruses encode various RNA silencing suppressors to counteract the antiviral silencing. Here, we identified p22 as a silencing suppressor of cucurbit chlorotic yellows crinivirus and showed that p22 interacts with CsSKP1LB1, a Cucumis sativus ortholog of S-phase kinase-associated protein 1 (SKP1). The F-box-like motif of p22 was identified through sequence analysis and found to be necessary for the interaction using a yeast two-hybrid assay. The involvement of the F-box-like motif in p22 silencing suppressor activity was determined. Proteomics analysis of Nicotiana benthamiana leaves expressing p22, and its F-box-like motif deletion mutant showed 228 differentially expressed proteins and five enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways: ABC transporters, sesquiterpenoid and triterpenoid biosynthesis, ubiquitin-mediated proteolysis, riboflavin metabolism, and cysteine and methionine metabolism. Collectively, our results demonstrate the interaction between p22 and CsSKP1LB1 and show that the deletion of F-box-like motif inhibits p22 silencing suppressor activity. The possible pathways regulated by the p22 through the F-box-like motif were identified using proteomics analysis.

SUBMITTER: Chen S

PROVIDER: S-EPMC6784205 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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Cucurbit Chlorotic Yellows Virus p22 Protein Interacts with Cucumber SKP1LB1 and Its F-Box-Like Motif Is Crucial for Silencing Suppressor Activity.

Chen Siyu S Sun Xinyan X Shi Yajuan Y Wei Ying Y Han Xiaoyu X Li Honglian H Chen Linlin L Sun Bingjian B Sun Hangjun H Shi Yan Y

Viruses 20190904 9

Plants use RNA silencing as a defense against viruses. In response, viruses encode various RNA silencing suppressors to counteract the antiviral silencing. Here, we identified p22 as a silencing suppressor of cucurbit chlorotic yellows crinivirus and showed that p22 interacts with CsSKP1LB1, a <i>Cucumis sativus</i> ortholog of S-phase kinase-associated protein 1 (SKP1). The F-box-like motif of p22 was identified through sequence analysis and found to be necessary for the interaction using a yea ...[more]

PMID: 31487883

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Project description:Background:Cucurbit aphid-borne yellows virus (CABYV) is among the most important yellowing viruses on cucurbits in Iran. The presence of CABYV has been previously reported from the major cucurbit growing areas in Iran, however, there are few studies concerning the detection of the different strains of this virus in the grower's fields, and especially, there is no report of the weed host plants near crop fields. Objectives: This study was done in order to detect the new strains of the CABYV polerovirus in cucurbits and the weed plants in the Lorestan province, Iran, as an introductory investigation for initiating a program of the breeding for resistance. Material and Methods: During a survey carried out in 2013-2014 in Lorestan province; Iran, 189 cucurbit and 261 weed samples were investigated for the presence of CABYV using RT-PCR. In addition, the phylogeny and nucleotide similarities were discussed on the basis of the partial nucleotide sequence of RNA dependent RNA polymerase (RdRP) gene. Results: The RT-PCR carried out on leaf samples revealed the infection with the CABYV in 43 cucumber and 12 weed samples. RT-PCR using strain specific primers detected the presence of the both common (C) and recombinant (R) strains of CABYV in the tested samples. On the basis of the phylogenetic analyses, the CABYV-C isolates from Iran were clustered into two distinct sub-populations (CI and CII), such that all the weed samples with two sequenced cucumber isolates were clustered in the CI sub-population. Meanwhile, a distinct sub-population of the isolates was clustered in the CABYV-R group showed a shared sequence identity of 97% to a Taiwanese isolate (JQ700306). Conclusions: This study has indicated the incidence of CABYV-R in the Southwest Asia; Iran for the first time. We were also able to show CABYV occurrence in Sysimbrium irio and Citrullus colocynthis from this area of the world. Identification of cucurbit infecting viruses and studying their distribution and potential reservoir hosts are important for developing successful control programs for virus disease management.

Dataset Information

Cucurbit Chlorotic Yellows Virus p22 Protein Interacts with Cucumber SKP1LB1 and Its F-Box-Like Motif Is Crucial for Silencing Suppressor Activity.

Publications

Cucurbit Chlorotic Yellows Virus p22 Protein Interacts with Cucumber SKP1LB1 and Its F-Box-Like Motif Is Crucial for Silencing Suppressor Activity.

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