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Integrating yeast chemical genomics and mammalian cell pathway analysis.

ABSTRACT: Chemical genomics has been applied extensively to evaluate small molecules that modulate biological processes in Saccharomyces cerevisiae. Here, we use yeast as a surrogate system for studying compounds that are active against metazoan targets. Large-scale chemical-genetic profiling of thousands of synthetic and natural compounds from the Chinese National Compound Library identified those with high-confidence bioprocess target predictions. To discover compounds that have the potential to function like therapeutic agents with known targets, we also analyzed a reference library of approved drugs. Previously uncharacterized compounds with chemical-genetic profiles resembling existing drugs that modulate autophagy and Wnt/?-catenin signal transduction were further examined in mammalian cells, and new modulators with specific modes of action were validated. This analysis exploits yeast as a general platform for predicting compound bioactivity in mammalian cells.

SUBMITTER: Zhou FL

PROVIDER: S-EPMC6786357 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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Integrating yeast chemical genomics and mammalian cell pathway analysis.

Zhou Fu-Lai FL Li Sheena C SC Zhu Yue Y Guo Wan-Jing WJ Shao Li-Jun LJ Nelson Justin J Simpkins Scott S Yang De-Hua DH Liu Qing Q Yashiroda Yoko Y Xu Jin-Biao JB Fan Yao-Yue YY Yue Jian-Min JM Yoshida Minoru M Xia Tian T Myers Chad L CL Boone Charles C Wang Ming-Wei MW

Acta pharmacologica Sinica 20190528 9

Chemical genomics has been applied extensively to evaluate small molecules that modulate biological processes in Saccharomyces cerevisiae. Here, we use yeast as a surrogate system for studying compounds that are active against metazoan targets. Large-scale chemical-genetic profiling of thousands of synthetic and natural compounds from the Chinese National Compound Library identified those with high-confidence bioprocess target predictions. To discover compounds that have the potential to functio ...[more]

PMID: 31138898

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Dataset Information

Integrating yeast chemical genomics and mammalian cell pathway analysis.

Publications

Integrating yeast chemical genomics and mammalian cell pathway analysis.

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