Recovery of genomes from metagenomes via a dereplication, aggregation and scoring strategy.

ABSTRACT: Microbial communities are critical to ecosystem function. A key objective of metagenomic studies is to analyse organism-specific metabolic pathways and reconstruct community interaction networks. This requires accurate assignment of assembled genome fragments to genomes. Existing binning methods often fail to reconstruct a reasonable number of genomes and report many bins of low quality and completeness. Furthermore, the performance of existing algorithms varies between samples and biotopes. Here, we present a dereplication, aggregation and scoring strategy, DAS Tool, that combines the strengths of a flexible set of established binning algorithms. DAS Tool applied to a constructed community generated more accurate bins than any automated method. Indeed, when applied to environmental and ho

SUBMITTER: Sieber CMK 

PROVIDER: S-EPMC6786971 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature