Development and Application of Sub-Mitochondrial Targeted Ca^{2 +} Biosensors.

ABSTRACT: Mitochondrial Ca²⁺ uptake into the mitochondrial matrix is a well-established mechanism. However, the sub-organellar Ca²⁺ kinetics remain elusive. In the present work we identified novel site-specific targeting sequences for the intermembrane space (IMS) and the cristae lumen (CL). We used these novel targeting peptides to develop green- and red- Ca²⁺ biosensors targeted to the IMS and to the CL. Based on their distinctive spectral properties, and comparable sensitivities these novel constructs were suitable to visualize Ca²⁺-levels in various (sub) compartments in a multi-chromatic manner. Functional studies that applied these new biosensors revealed that knockdown of MCU and EMRE yielded elevated Ca²⁺ levels inside the CL but not the

SUBMITTER: Waldeck-Weiermair M 

PROVIDER: S-EPMC6788349 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature