Ontology highlight
ABSTRACT:
SUBMITTER: Wang Q
PROVIDER: S-EPMC6794629 | biostudies-literature | 2019 Oct
REPOSITORIES: biostudies-literature
Wang Qixue Q Liu Xing X Zhou Junhu J Yang Chao C Wang Guangxiu G Tan Yanli Y Wu Ye Y Zhang Sijing S Yi Kaikai K Kang Chunsheng C
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 20190829 20
RNA is rarely used as a therapeutic target due to its flexible structure and instability. CRISPR-Cas13a is a powerful tool for RNA knockdown, and the potential application of CRISPR-Cas13a in cancer cells should be further studied. In this study, overexpression of LwCas13a by lentivirus in glioma cells reveals that crRNA-EGFP induces a "collateral effect" after knocking down the target gene in EGFP-expressing cells. EGFRvIII is a unique EGFR mutant subtype in glioma, and the CRISPR-Cas13a system ...[more]