Ontology highlight
ABSTRACT:
SUBMITTER: Aman R
PROVIDER: S-EPMC5755456 | biostudies-literature | 2018 Jan
REPOSITORIES: biostudies-literature
Genome biology 20180104 1
<h4>Background</h4>CRISPR/Cas systems confer immunity against invading nucleic acids and phages in bacteria and archaea. CRISPR/Cas13a (known previously as C2c2) is a class 2 type VI-A ribonuclease capable of targeting and cleaving single-stranded RNA (ssRNA) molecules of the phage genome. Here, we employ CRISPR/Cas13a to engineer interference with an RNA virus, Turnip Mosaic Virus (TuMV), in plants.<h4>Results</h4>CRISPR/Cas13a produces interference against green fluorescent protein (GFP)-expre ...[more]