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Synthesis and Enzymatic Incorporation of a Responsive Ribonucleoside Probe That Enables Quantitative Detection of Metallo-Base Pairs.

ABSTRACT: Synthesis of a highly responsive fluorescent ribonucleoside analogue based on a 5-methoxybenzofuran uracil core, enzymatic incorporation of its triphosphate substrate into RNA transcripts, and its utility in the specific detection and estimation of Hg2+-ion-mediated metallo-base pair formation in DNA-RNA and RNA-RNA duplexes are described.

SUBMITTER: Manna S

PROVIDER: S-EPMC6794643 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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Synthesis and Enzymatic Incorporation of a Responsive Ribonucleoside Probe That Enables Quantitative Detection of Metallo-Base Pairs.

Manna Sudeshna S Srivatsan Seergazhi G SG

Organic letters 20190607 12

Synthesis of a highly responsive fluorescent ribonucleoside analogue based on a 5-methoxybenzofuran uracil core, enzymatic incorporation of its triphosphate substrate into RNA transcripts, and its utility in the specific detection and estimation of Hg<sup>2+</sup>-ion-mediated metallo-base pair formation in DNA-RNA and RNA-RNA duplexes are described. ...[more]

PMID: 31184159

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